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位置: 首页 > ELISA试剂盒 > Human Free Thyroxine (fT4) ELISA Kit

Human Free Thyroxine (fT4) ELISA Kit#EK11594

是否有货: 1-2周
产品总价:
产品详情

产品名称Human Free Thyroxine (fT4) ELISA Kit

简述ELISA Kit

应用ELISA

种属反应性Human (Homo sapiens)

保存The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37C for 4 and 7 days, and compare O.D.values of the kit kept at 37C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37C can be considered as 6 months at 2 - 8C, which means 7 days at 37C equaling 12 months at 2 - 8C).

应用详情

Detect Range:4- 64 pmol/L
Sensitivity:1.08 pmol/L
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 μL
Assay Time:1-4.5h
Detection wavelength:450 nm

产品描述

This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for fT4 has been pre-coated onto a microplate. Standards or samples and a biotin-conjugated antibody specific for fT4 are pipetted into the wells. A competitive inhibition reaction is launched between fT4 (Standards or samples) and biotin-conjugated fT4 with the pre-coated antibody specific for fT4. After washing, streptavidin conjugated Horseradish Peroxidase (HRP) is added
to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a
substrate solution is added to the wells and color develops in proportion to the
amount of fT4 bound in the initial step. The color development is stopped and the
intensity of the color is measured.

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5

注释

应用

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  • DB免疫斑点法
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  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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