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位置: 首页 > 磷酸化抗体 > Rb(Phospho-Ser780) Antibody

Rb(Phospho-Ser780) Antibody#11132

Rb(Phospho-Ser780) Antibody
Rb(Phospho-Ser780) Antibody
Rb(Phospho-Ser780) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Rb(Phospho-Ser780) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Rb only when phosphorylated at serine 780.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 780 (T-L-S(p)-P-I) derived from Human Rb.

基因/蛋白名称Rb

修饰Phospho

别名P105-RB; PP105; PP110; RB-1; RB1

数据库入口号Swiss-Prot: P06400
NCBI Protein: NP_000312.2

UniprotP06400

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 110kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from 3T3 cells untreated or treated with UV using Rb(Phospho-Ser780) Antibody #11132.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rb(Phospho-Ser780) Antibody #11132(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using Rb(Phospho-Ser780) Antibody #11132.
背景
Key regulator of entry into cell division that acts as a tumor suppressor. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.

Roesch A, et al.(2005) Mod Pathol. 18(4): 565-572.
Huang H, et al.(2004)Oncogene. 23(12): 2161-2176.
Kitagawa M, et al.(1996)EMBO J. 15(24): 7060-7069.

如果您使用该产品11132发表了文章,请通知我们,让我们可以引用您的文献。

et al,The potent novel CDK4/6 inhibitor TQB3616 in hormone receptor positive breast cancer: preclinical characterization with in vitro and human tumor xenograft modelsInBreast Cancer?On2023 Dec 8byWenyu Hu?#?1,?Lei Wang et al..PMID:?38090281, , (2023),
PMID: 38090281
et al,Palbociclib inhibits epithelial-mesenchymal transition and metastasis in breast cancer via c-Jun/COX-2 signaling pathway.In Oncotarget on 2015 Dec 8 by Ge Qin, Fei Xu et al..PMID: 26540629, , (2015),
PMID: 26540629
Xueting Cai, Tingmei Ye, Chao Liu et al,Luteolin induced G2 phase cell cycle arrest and apoptosis on non-small cell lung cancer cells., Toxicology in Vitro, 25(7):1385-1391 (2011),
PMID: 21601631
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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