Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate fMET in samples. An antibody specific for fMET has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyfMET present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for fMET is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of fMET bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:fMet-Leu-Phe receptor is a protein encoded by the?FPR1?gene.Ozcelik et al. mapped the FPR1 gene to human chromosome 19 by Southern analysis of rodent/human somatic cell hybrids and use of a cDNA probe. An FPR-like gene was also identified on chromosome 19 . An FPR-like gene that mapped to chromosome 2 was found in fact to represent an interleukin 8 receptor. Bao et al. mapped the gene encoding the receptor for the chemotactic ligand FMLP to chromosome 19, using a panel of somatic cell hybrids. They identified 2 structural homologs of FPR that similarly mapped to chromosome 19. The structural homologs did not recognize the ligand FMLP, but were thought to represent chemotactic receptors. Bao et al. referred to them as orphan receptors and symbolized the genes FPRH1 and FPRH2.