Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate AMH in samples. An antibody specific for AMH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAMH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Anti-Mllerian hormone (AMH) is a dimeric glycoprotein that inhibits the development of the Mllerian ducts in a male embryo. It is named after Johannes Peter Mller. AMH is a protein hormone structurally related to inhibin and activin, and a member of the transforming growth factor-β (TGF-β) family. It is present in fish, reptiles, birds, marsupials, and placental mammals.In mammals AMH is secreted by Sertoli cells of the testes during embryogenesis of the fetal male and prevents the development of the mullerian ducts into the uterus and other mullerian structures. The effect is ipsilateral, that is each testis suppresses Mllerian development only on its own side. In humans this action takes place by 8 weeks gestation. In female embryogenesis the absence of AMH allows for the development of upper vagina, uterus and cervix, and oviducts.