Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate ECP in samples. An antibody specific for ECP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyECP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ECP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ECP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Three immunization procedures were compared for the production of antibodies to the minor components of a complex E. coli protein (ECP) mixture: a conventional protocol and two methods that allow for the selective in vitro (cascade) or in vivo (passive) depletion of highly immunogenic proteins.An E. coli protein, designated , has been purified at least 1000-fold. Treatment of a eovalently closed DNA duplex containing negative superhelical turns with results in the loss of most of the superhelical turns. The loss of superhelical turns follows a gradual course rather than a one-hit mechanism. This reaction does not require a cofactor. No other change in the physical properties of the DNA could be detected. is capable of introducing a swivel reversibly into a DNA. A plausible mechanism is postulated.