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位置: 首页 > 磷酸化抗体 > CDK2(Phospho-Thr160) Antibody

CDK2(Phospho-Thr160) Antibody#11133

CDK2(Phospho-Thr160) Antibody
CDK2(Phospho-Thr160) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称CDK2(Phospho-Thr160) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of CDK2 only when phosphorylated at threonine 160.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 160 (T-Y-T(p)-H-E) derived from Human CDK2.

基因/蛋白名称CDK2

修饰Phospho

别名kinase Cdk2; p33 protein kinase;

数据库入口号Swiss-Prot: P24941
NCBI Protein: NP_001789.2

UniprotP24941

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 34kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDK2(Phospho-Thr160) Antibody #11133.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDK2(Phospho-Thr160) Antibody #11133(left) or the same antibody preincubated with blocking peptide(right).
背景
Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.

Ukomadu C, et al.(2003) J Biol Chem; 278(7): 4840-6.
Morris MC, et al.(2002)J Biol Chem; 277(26): 23847-53.
Brown NR, et al.(1999)J Biol Chem; 274(13): 8746-56.
Liu Y, et al.(2004) J Biol Chem; 279(6): 4507-14.

如果您使用该产品11133发表了文章,请通知我们,让我们可以引用您的文献。

et al,Pro-Apoptotic Effects of JDA-202, a Novel Natural Diterpenoid, on Esophageal Cancer Through Targeting Peroxiredoxin I.In Antioxid Redox Signal on 2017 Jul 10 by Xiao-Jing Shi , Lina Ding,et al..PMID: 27650197, , (2017),
PMID: 27650197
et al,Jaridonin-induced G2/M Phase Arrest in Human Esophageal Cancer Cells Is Caused by Reactive Oxygen Species-Dependent Cdc2-tyr15 Phosphorylation via ATM-Chk1/2-Cdc25C Pathway .In Toxicol Appl Pharmacol on 2015 Jan 15 by Yong-Cheng Ma , Nan Su et al..PMID:25450480, , (2015),
PMID: 25450480
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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