产品详情
产品名称L1 Polyclonal Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Caprylic Acid Ammonium Sulfate Precipitation purified
应用WB IHC
种属反应性virus
特异性The antibody detects endogenous level of total L1 polyclonal antibody.
免疫原类型protein
免疫原描述Recombinant HPV18 Major capsid protein L1 protein
基因/蛋白名称L1
别名L1
数据库入口号Swiss-Prot#: P06794
UniprotP06794
计算分子量62kd
配方Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
保存Store at -20˚C
应用详情
Western blotting: 1:500 - 1:1000
背景
Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on the basement membrane to provide initial virion attachment to target cells. Basement membrane is exposed only after epithelium trauma. Additionally, the alpha6 integrin complexed with either beta1 or beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, integrin complexed with beta4 integrin has been proposed to act as a coreceptor recognized by L1. Once attached, the virion enters the host cell via clathrin-mediated endocytosis and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.
背景文献
[1] "Human papillomavirus type 16 DNA sequence."Seedorf K., Krammer G., Durst M., Suhai S., Rowekamp W.G.Virology 145:181-185(1985) [2] "Nucleotide sequences and further characterization of human papillomavirus DNA present in the CaSki, Si