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位置: 首页 > 磷酸化抗体 > FKHR(Phospho-Ser319) Antibody

FKHR(Phospho-Ser319) Antibody#11136

FKHR(Phospho-Ser319) Antibody
FKHR(Phospho-Ser319) Antibody
FKHR(Phospho-Ser319) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称FKHR(Phospho-Ser319) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of FKHR only when phosphorylated at serine 319.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 319 (T-S-S(p)-N-A) derived from Human FKHR/FOXO1A.

基因/蛋白名称FKHR

修饰Phospho

别名 FOXO1

数据库入口号Swiss-Prot: Q12778
NCBI Protein: NP_002006.2

UniprotQ12778

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 78-82 kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from 3T3 cells untreated or treated with serum starvation using FKHR(Phospho-Ser319) Antibody #11136.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FKHR(Phospho-Ser319) Antibody #11136(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using FKHR(Phospho-Ser319) Antibody #11136.
背景

FKHR belongs to the forkhead family of transcription factors, which are characterized by a distinct forkhead domain. It may play a role in myogenic growth and differentiation. The mammalian DAF-16-like transcription factors, FKHR, FKHRL1, and AFX, function as key regulators of insulin signaling, cell cycle progression, and apoptosis downstream of phosphoinositide 3-kinase. Gene activation through binding to insulin response sequences has been essential for mediating these functions. D-type Cyclins (in Class III) is required for FKHR mediated inhibition of cell cycle progression and transformation. FKHR gene is mapped to chromosome 13q14

Rena G, et al. (2002) EMBO J 21(9): 2263-2271.
Woods YL, et al. (2001) Biochem J355(Pt 3): 597-607.
Rena G, et al. (2001) Biochem J 354(Pt 3): 605-612.

如果您使用该产品11136发表了文章,请通知我们,让我们可以引用您的文献。

et al,Translocation of forkhead box O1 to the nuclear periphery induces histone modifications that regulate transcriptional repression of PCK1 in HepG2 cells.In Genes Cells on 2015 Apr by Tamaki Arai, Fumi Kano et al..PMID: 25736587, , (2015),
PMID: 25736587
et al,The nitric oxide-cyclic GMP pathway regulates FoxO and alters dopaminergic neuron survival in Drosophila. In PLoS One on 2012 by Tomoko Kanao, Tomoyo Sawada, et al..PMID:22393355, , (2012),
PMID: 22393355
et al,Activation of FoxO by LRRK2 induces expression of proapoptotic proteins and alters survival of postmitotic dopaminergic neuron in Drosophila. In Hum Mol Genet on 2010 Oct 1 by Tomoko Kanao, Katerina Venderova, et al..PMID:20624856, , (2010),
PMID: 20624856
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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