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位置: 首页 > 磷酸化抗体 > cdc25A(Phospho-Ser76) Antibody

cdc25A(Phospho-Ser76) Antibody#11138

cdc25A(Phospho-Ser76) Antibody
cdc25A(Phospho-Ser76) Antibody
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产品总价:
产品详情

产品名称cdc25A(Phospho-Ser76) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of cdc25A only when phosphorylated at serine 76.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 76 (M-G-S(p)-S-E) derived from Human cdc25A.

基因/蛋白名称cdc25A

修饰Phospho

别名CDC25M3; MPIP1;

数据库入口号Swiss-Prot: P30304
NCBI Protein: NP_001780.2

UniprotP30304

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 70kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela cells untreated or treated with TNF-a and CA using cdc25A(Phospho-Ser76) Antibody #11138.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A(Phospho-Ser76) Antibody #11138(left) or the same antibody preincubated with blocking peptide(right).
背景
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

Jin J, et al. (2003) Genes 17(24): 3062-74.
Shimuta K, et al. (2002) EMBO J 21(14): 3694-703.

如果您使用该产品11138发表了文章,请通知我们,让我们可以引用您的文献。

Shunfu Piao, Su-Jin Lee, Yongbin Xu et al,CK1娄脜 targets Cdc25A for ubiquitin-mediated proteolysis under normal conditions and in response to checkpoint activation, Cell Cycle, 10(3):531-537 (2011),
PMID: 21252624
et al,CK1蔚 targets Cdc25A for ubiquitin-mediated proteolysis under normal conditions and in response to checkpoint activation. In Cell Cycle on 2011 Feb 1 by Shunfu Piao, Su-Jin Lee, et al..PMID: 21252624, , (2011),
PMID: 21252624
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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