产品详情
产品名称cofilin(Phospho-Ser3) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level of cofilin only when phosphorylated at serine 3.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin.
基因/蛋白名称cofilin
修饰Phospho
别名CFL; CFL1;
数据库入口号Swiss-Prot: P23528
NCBI Protein: NP_005498.1
UniprotP23528
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 19kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from COLO205 cells using cofilin(Phospho-Ser3) Antibody #11139(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin(Phospho-Ser3) Antibody #11139(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using cofilin(Phospho-Ser3) Antibody #11139.
Lane 1 : Hela treated with H2O2;
Lane 2 : Mouse brain tissue;
Lane 3 : Rat brain tissue;
Lane 4 : N-peptide blocked Hela treated with H2O2;
Lane 5 : N-peptide blocked Mouse brain tissue;
Lane 6 : N-peptide blocked Rat brain tissue;
Lane 7 : P-peptide blocked Hela treated with H2O2;
Lane 8 : P-peptide blocked Mouse brain tissue;
Lane 9 : P-peptide blocked Rat brain tissue;
Lysates/proteins at 40 µg per lane.
Predicted band size : 19 kDa
Observed band size : 19 kDa
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