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位置: 首页 > 重组蛋白 > Sequencing Grade Chymotrypsin

Sequencing Grade Chymotrypsin#RE013

是否有货: Yes
产品总价:
产品详情

产品名称Sequencing Grade Chymotrypsin

纯化SDS-PAGE:≥90%

种属反应性(unit /mg protein) ≥1500 U/mg protein

CAS号9004-07-3 EC: 3.4.2.1

序列The amino acid sequence of sequencing grade chymotrypsin is identical to human chymotrypsin

配置The Sequencing Grade Chymotrypsin lyophilized powder was dissolved into 0.5-1 ug/uL using resolution buffer. Use 50 mM NH4HCO3 or PH 7.0-8.0 buffer solution. It is recommended to use 1 mM CaCl2 in the digestion buffer, recombinant sequenced grade chymotrypsin: target protein = 1:2000-1:20.

保存Stability of storage:Sequencing grade chymotrypsin lyophilized should be stored under 2-8˚C in sealed container. It is stable within 24 months. Use resolution buffer to dissolve the lyophilized powder into 0.5-1 ug/uL, and store it at -20±5℃ after aliquote it to avoid repeated freeze-thaw. Stability of transport: The product is stable by blue ice insulation transport.

产品描述

Sequencing grade chymotrypsin is a recombinant serine endopeptidase expressed in Pichia pastoris, purified with HPLC, the gene sequence is the same as human chymotrypsin. Sequencing grade chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. The optimal pH is 7.0–9.0.

背景

Recombinant Sequencing Grade Chymotrypsin is expressed using the expression system of E.coli, serine endopeptidase, whose amino acid sequence is identical to that of human chymotrypsin.The optimal pH of Recombinant Sequencing Grade Chymotrypsin was 7.0-9.0. Applicable to the cleavage of the following specific sites in protein sequencing: Tyr┼, Trp┼, Phe┼, Leu┼.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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