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Western blot analysis of NNMT expression in Hela (A), HepG2 (B), mouse brain (C), rat lung (D), rat liver (E) whole cell lysates.
Immunohistochemical analysis of NNMT staining in human
liver formalin fixed paraffin embedded tissue section. The
section was pre-treated using heat mediated antigen retrieval
with sodium citrate buffer (pH 6.0). The section was then
incubated with the antibody at room temperature and
detected using an HRP conjugated compact polymer system.
DAB was used as the chromogen. The section was then
counterstained with haematoxylin and mounted with DPX
Immunofluorescent analysis of NNMT staining in Hela cells.
Formalin-fixed cells were permeabilized with 0.1% Triton
X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS
for 30 minutes at room temperature. Cells were probed with
the primary antibody in 3% BSA-PBS and incubated overnight
at 4 °C in a hidified chamber. Cells were washed with PBST and
incubated with a DyLight 594-conjugated secondary antibody
(red) in PBS at room temperature in the dark.
