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位置: 首页 > 抗体 > 14-3-3 beta antibody

14-3-3 beta antibody#22832

14-3-3 beta antibody
14-3-3 beta antibody
14-3-3 beta antibody
是否有货: 有货
产品总价:
产品详情

产品名称14-3-3 beta antibody

来源种属Rabbit

克隆性Polyclonal

纯化Purified by antigen-affinity chromatography.

应用WB IHC IF

种属反应性Hu

免疫原类型Recombinant protein

免疫原描述Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 141 of 14-3-3 beta

基因/蛋白名称14-3-3b

数据库入口号NCBI Gene ID: 7529
NCBI mRNA#: BC001359
NCBI Protein#: AAH01359

浓度1mg/ml

配方Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

保存Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

应用详情
Predicted MW: 28kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:500
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: Hela
D: Hep G2
E: Molt-4
F: Raji
12% SDS PAGE
Primary antibody diluted at 1: 10000
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using 14-3-3 beta antibody at 1: 500 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta antibody at 1: 200 dilution.
背景
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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025-58868422

Email Address

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