免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 磷酸化抗体 > PDHK1(Phospho-Tyr243) Antibody

PDHK1(Phospho-Tyr243) Antibody#11597

PDHK1(Phospho-Tyr243) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称PDHK1(Phospho-Tyr243) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Rt

特异性The antibody detects endogenous level of PDHK1 only when phosphorylated at tyrosine 243.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 243(D-L-Y(p)-Y-I) derived from Human PDHK1.

基因/蛋白名称PDHK1

修饰Phospho

别名PDK1; PDH kinase 1

数据库入口号Swiss-Prot: Q15118
NCBI Protein: NP_001265478.1

UniprotQ15118

目标蛋白种属Human

计算分子量49kd

浓度1.0mg/mL

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Western blotting: 1:500~1:1000 

U87 cells were stimulated with or without hypoxia for 6 h. Immunoblotting analyses of mitochondrial lysates were performed with the indicated antibodies
背景
 Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.
 
Gudi R., Bowker-Kinley M.M., Kedishvili N.Y., Zhao Y., Popov K.M.J. Biol. Chem. 270:28989-28994(1995)
The MGC Project TeamGenome Res. 14:2121-2127(2004)

 

如果您使用该产品11597发表了文章,请通知我们,让我们可以引用您的文献。

et al,Pyruvate dehydrogenase inactivation causes glycolytic phenotype in BAP1 mutant uveal melanoma. In Oncogene on 2022 Feb by Anna Han, Vivian Chua,et al..PMID:35046531, , (2022),
PMID: 35046531
Li X, et al. et al,Mitochondria-Translocated PGK1 Functions as a Protein Kinase to Coordinate Glycolysis and the TCA Cycle in Tumorigenesis., Mol Cell., 3;61(5):705-19. (2016 Mar),
PMID: 26942675
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com