产品详情
产品名称VRK1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antigen affinity purification
应用IHC WB
种属反应性Hu
特异性The antibody detects endogenous levels of total VRK1 protein.
免疫原类型protein
免疫原描述Fusion protein of human VRK1
基因/蛋白名称VRK1
别名PCH1; PCH1A
数据库入口号Swiss-Prot#: Q99986
NCBI Gene ID: 7443
UniprotQ99986
计算分子量45kd
浓度0.8mg/ml
配方Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:200-1000
Immunohistochemistry: 1: 20-100
Gel: 8%SDS-PAGE
Lysate: 40 μg, Lane 1-4: Jurakt,Hela,293T and 231 cell lysates,
Primary antibody:VRK1 antibody at dilution 1/400,
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
Exposure time: 5 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using VRK1 Antibody at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
背景
This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.?
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