产品详情
产品名称JAK1(Phospho-Tyr1022) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Hu Ms Rt
特异性The antibody detects endogenous level of JAK1 only when phosphorylated at tyrosine 1022.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of tyrosine 1022 (K-E-Y(p)-Y-T) derived from Human JAK1.
基因/蛋白名称JAK1
修饰Phospho
别名 Janus kinase 1
数据库入口号Swiss-Prot: P23458
NCBI Protein: NP_002218.2
UniprotP23458
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 130kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
IF 1:50 - 1:200
Western blot analysis of extracts from HT29 cells untreated or treated with UV using JAK1(Phospho-Tyr1022) Antibody #11149.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using JAK1(Phospho-Tyr1022) Antibody #11149(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence analysis of mouse-liver tissue. JAK1 (phospho Tyr1022) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight).
Immunofluorescence analysis of mouse-liver tissue. 1,JAK1 (phospho Tyr1022) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight).
如果您使用该产品11149发表了文章，请通知我们，让我们可以引用您的文献。
et al,A 2-Benzylmalonate Derivative as STAT3 Inhibitor Suppresses Tumor Growth in Hepatocellular Carcinoma by Upregulating β-TrCP E3 Ubiquitin Ligase. In Int J Mol Sci on 2021
Mar 25 by Ting Peng, Orawan Wonganan, et al..PMID:33805945,
, (2021),
PMID: 33805945
et al,Salinomycin promotes T-cell proliferation by inhibiting the expression and enzymatic activity of immunosuppressive indoleamine-2,3-dioxygenase in human breast cancer cells. In Toxicol Appl Pharmacol on 2020 Oct 1 by Yuwen Sheng, Zhonghui Zhang, et al..PMID:32822738,
, (2020),
PMID: 32822738
et al,A thiazole-derived oridonIn analogue exhibits antitumor activity by directly and allosterically InhibitIng STAT3. In J Biol Chem on 2019 Nov 15;by Shen X, Zhao L,et al..PMID:31594861,
, (2019),
PMID: 31594861
et al,Activation of Janus kinase 1 confers poor prognosis in patients with non鈥憇mall cell lung cancer.In Oncol Lett on 2017 Oct by Dan Liu, Yi Huang ,et al..PMID: 28989534,
, (2017),
PMID: 28989534
et al,Notch-induced hIL-6 production facilitates the maintenance of self-renewal of hCD34+ cord blood cells through the activation of Jak-PI3K-STAT3 pathway. In Am J Pathol
on 2012 Jan by Bongkum Choi, Eunyoung Chun, et al..PMID:22062221,
, (2012),
PMID: 22062221
et al,Down-regulation of JAK1 by RNA interference inhibits growth of the lung cancer cell line A549 and interferes with the PI3K/mTOR pathway. In J Cancer Res Clin Oncol
on 2011 Nov by Dan Liu, Yi Huang, et al..PMID:21861134,
, (2011),
PMID: 21861134
YOUNG CHA, BO-HYUN MOON, MI-OK LEE et al,Zap70 Functions to Maintain Stemness of Mouse Embryonic Stem Cells by Negatively Regulating Jak1/Stat3/c-Myc Signaling.,
STEM CELLS., 28(9):1476篓C1486 (2010),
PMID: 20641039
查看更多
et al,Zap70 functions to maintain stemness of mouse embryonic stem cells by negatively regulating Jak1/Stat3/c鈥怣yc signaling.In Stem Cells on 2010 Sep by Young Cha ,
Bo-hyun Moon, et al..PMID:20641039,
, (2010),
PMID: 20641039
有货
