产品详情
产品名称IkB-a(Phospho-Ser32/Ser36) Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
应用WB IHC IF
种属反应性Hu Ms
特异性The antibody detects endogenous level of IkB-a only when phosphorylated at serine 32/36.
免疫原类型Peptide-KLH
免疫原描述Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IkB-a.
基因/蛋白名称IkB-a
修饰Phospho
别名I-kappa-B-alpha; IKBA; NF-kappaB inhibitor alpha; NFKBI; NFKBIA
数据库入口号Swiss-Prot: P25963
NCBI Protein: NP_065390.1
UniprotP25963
浓度1.0mg/ml
配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
应用详情
Predicted MW: 39kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
背景
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to tranlocate to the nucleus and activate transcription.
Shieh, S. Y. et al. (1999) EMBO J. 18, 1815-1823.
Honda, R. et al. (1997) FEBS Lett. 420, 25-27.
Hirao, A. et al. (2000) Science 287, 1824-1827.
Tibbetts, R. S. et al. (1999) Genes Dev. 13, 152-157.
Chehab,N.H.et al. (1999) Proc .Natl .Acad. Sci. USA 96, 13777-13782.
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