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位置: 首页 > 磷酸化抗体 > Src(Phospho-Tyr529) Antibody

Src(Phospho-Tyr529) Antibody#11153

Src(Phospho-Tyr529) Antibody
Src(Phospho-Tyr529) Antibody
Src(Phospho-Tyr529) Antibody
Src(Phospho-Tyr529) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Src(Phospho-Tyr529) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Src only when phosphorylated at tyrosine 529.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 529 (P-Q-Y(p)-Q-P) derived from Human Src.

基因/蛋白名称Src

修饰Phospho

别名C-SRC; SRC1;

数据库入口号Swiss-Prot: P12931
NCBI Protein: NP_005408.1

UniprotP12931

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 60kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HT29 cells untreated or treated with PMA using Src(Phospho-Tyr529) Antibody #11153.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Src(Phospho-Tyr529) Antibody #11153(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using Src(Phospho-Tyr529) Antibody #11153.
Western blot analysis of extracts from HepG2 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using Src (Phospho-Tyr529) Antibody #11153.
背景

proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme.

Pyper J.M., (1985) Mol. Cell. Biol. 5:831-838
Pyper J.M.(1990) Mol. Cell. Biol. 10:2035-2040
Xu W., (1997).Nature 385:595-602
Benes C.H., (2005) Cell 121:271-280

如果您使用该产品11153发表了文章,请通知我们,让我们可以引用您的文献。

et al,Identification of Demethylincisterol A 3 as a Selective Inhibitor of Protein Tyrosine Phosphatase Shp2.In Eur J Pharmacol on 2017 Jan 15 by Chuan Chen , Fan Liang,et al..PMID: 27939989, , (2017),
PMID: 27939989
et al,Discovery of a novel inhibitor of the protein tyrosine phosphatase Shp2.In Sci Rep on 2015 Dec 2 by Chuan Chen , Mengmeng Cao et al..PMID: 26626996 , , (2015),
PMID: 26626996
et al,Neuronal deletion of GSK3尾 increases microtubule speed in the growth cone and enhances axon regeneration via CRMP-2 and independently of MAP1B and CLASP2.In MC Biol on 2014 Jun 12 by M谩rcia A Liz, Fernando M Mar et al..PMID: 24923837, , (2014),
PMID: 24923837
Yanhua Zheng, Yan Xia, Xiang Gao et al,FAK Phosphorylation by ERK Primes Ras-Induced Tyrosine Dephosphorylation of FAK Mediated by PIN1 and PTP-PEST., Molecular Cell, 35(1):11篓C25 (2009),
PMID: 19595712
et al,FAK phosphorylation by ERK primes ras-induced tyrosine dephosphorylation of FAK mediated by PIN1 and PTP-PEST. In Mol Cell on 2009 Jul 10 by Yanhua Zheng, Yan Xia, et al..PMID:19595712, , (2009),
PMID: 19595712
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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