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Cytokeratin 20 antibody#22920

Cytokeratin 20 antibody
Cytokeratin 20 antibody
Cytokeratin 20 antibody
是否有货: 有货
产品总价:
产品详情

产品名称Cytokeratin 20 antibody

来源种属Rabbit

克隆性Polyclonal

纯化Purified by antigen-affinity chromatography.

应用WB IHC IF

种属反应性Hu

免疫原类型Recombinant protein

免疫原描述Recombinant protein fragment contain a sequence corresponding to a region within amino acids 75 and 287 of Cytokeratin 20

基因/蛋白名称Cytokeratin 20

数据库入口号NCBI Gene ID: 54474
NCBI mRNA#: NM_019010
NCBI Protein#: NP_061883

浓度1mg/ml

配方Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

保存Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

应用详情
Predicted MW: 49kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:100-1:500
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
Primary antibody diluted at 1: 3000
Immunohistochemical analysis of paraffin-embedded HT29 xenograft, using Cytokeratin 20 antibody at 1: 100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cytokeratin 20 antibody at 1: 200 dilution.
背景
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq]

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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