产品详情
产品名称WDSUB1 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antigen affinity purification
应用IHC WB
种属反应性Hu
特异性The antibody detects endogenous levels of total WDSUB1 protein.
免疫原类型peptide
免疫原描述Synthetic peptide of human WDSUB1
基因/蛋白名称WDSUB1
别名UBOX6; WDSAM1
数据库入口号Swiss-Prot#: Q8N9V3
NCBI Gene ID: 151525
UniprotQ8N9V3
计算分子量53kd
浓度0.6mg/ml
配方Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol.
保存Store at -20˚C
应用详情
Western blotting: 1:200-1000
Immunohistochemistry: 1: 20-100
Gel: 8%SDS-PAGE
Lysate: 40 μg, Lane 1-4: 231£¬K562£¬293T and Hela cell lysates,
Primary antibody:WDSUB1 antibody at dilution 1/250,
Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,
Exposure time: 10 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using WDSUB1 Antibody at dilution 1/25, on the right is treated with synthetic peptide. (Original magnification: x200)
背景
WDSUB1 (WD repeat, SAM and U-box domain-containing protein 1), also known as UBOX6 or WDSAM1, is a 476 amino acid protein that contains one SAM (sterile alpha motif) domain, one U-box domain and seven WD repeats. Existing as two isoforms due to alternative splicing, WDSUB1 is encoded by a gene located on chromosome 2. The second largest human chromosome, chromosome 2 encodes over 1,400 genes and comprises nearly 8% of the human genome, housing a number of disease-associated genes. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the ABCA12 gene, while the lipid metabolic disorder sitosterolemia is associated with defects in the ABCG5 and ABCG8 genes. Additionally, an extremely rare recessive genetic disorder, Alstr?m syndrome, is caused by mutations in the ALMS1 gene, which maps to chromosome 2.
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