免费电话:400-9961-933
邮箱:techcn@signalwayantibody.com
欢迎光临本站
登录 注册 English
位置: 首页 > 磷酸化抗体 > Zap-70(Phospho-Tyr319) Antibody

Zap-70(Phospho-Tyr319) Antibody#11159

Zap-70(Phospho-Tyr319) Antibody
Zap-70(Phospho-Tyr319) Antibody
Zap-70(Phospho-Tyr319) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称Zap-70(Phospho-Tyr319) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of VEGFR2 only when phosphorylated at tyrosine 1214.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 319 (S-P-Y(p)-S-D) derived from Human Zap-70.

基因/蛋白名称Zap-70

修饰Phospho

别名SRK, STD, TZK, STCD, ZAP-70

数据库入口号Swiss-Prot: P43403
NCBI Protein: NP_001070.2

UniprotP43403

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 70kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from Jurkat cells using Zap-70(Phospho-Tyr319) Antibody #11159(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunofluorescence staining of methanol-fixed Hela cells using Zap-70(Phospho-Tyr319) Antibody #11159.
Western blot analysis of extracts from HL60 cells, treated with calf intestinal phosphatase (CIP), using Zap-70 (Phospho-Tyr319) Antibody #11159.
背景

Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

Salomon AR, et al. (2003) Proc Natl Acad Sci U S A; 100(2): 443-448
Ku GM, et al. (2001) EMBO J; 20(3): 457-465
Tang J, et al. (1999) Proc Natl Acad Sci U S A; 96(17): 9775-9780
Zhao Q, et al. (1996) Mol Cell Biol; 16(12): 6765-6774
Williams BL, et al. (1999) EMBO J; 18(7): 1832-1844
Adjali O, et al. (2005) J Clin Invest; 115(8): 2287-2295.

如果您使用该产品11159发表了文章,请通知我们,让我们可以引用您的文献。

5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

Location :

南京市高新开发区星火路10号人才大厦C座

Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com