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位置: 首页 > 磷酸化抗体 > RAD50 (Phospho-Ser635) Antibody

RAD50 (Phospho-Ser635) Antibody#12632

RAD50 (Phospho-Ser635) Antibody
RAD50 (Phospho-Ser635) Antibody
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产品详情

产品名称RAD50 (Phospho-Ser635) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB;IHC

种属反应性Hu Ms Rt

特异性RAD50 (Phospho-Ser635) Antibody detects endogenous levels of RAD50 only when phosphorylated at Ser635

免疫原类型Peptide

免疫原描述A synthesized peptide derived from human RAD50 (Phospho-Ser635)

基因/蛋白名称RAD50

修饰Phospho

别名RAD50, DNA repair protein RAD50, HRad50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2

数据库入口号Swiss-Prot#: Q92878
NCBI Gene ID: 10111

UniprotQ92878

目标蛋白种属human

计算分子量154kd

浓度1.0mg/ml

配方Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

WB 1:500-2000;
IHC-p 1:50-300

Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min)
Western Blot analysis of various cell lysates, using primary antibody at 1:500 dilution. Secondary antibody was diluted at 1:10000

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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