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DFFA Polyclonal Antibody#27370

DFFA Polyclonal Antibody
DFFA Polyclonal Antibody
DFFA Polyclonal Antibody
是否有货: 货期一周
产品总价:
产品详情

产品名称DFFA Polyclonal Antibody

来源种属Rabbit

克隆性Polyclonal

亚型IgG

纯化Affinity purification

应用WB,IF

种属反应性Hu,Ms,Rt

免疫原描述Recombinant fusion protein of human DFFA (NP_004392.1).

别名DFFA; DFF-45; DFF1; ICAD; DNA fragmentation factor subunit alpha

数据库入口号Swiss-Prot#:O00273
NCBI Gene ID:1676

计算分子量35kDa, 45kDa

配方Avoid freeze / thaw cycles.|Buffer: PBS with 50% glycerol, pH7.4.

保存Store at -20˚C

应用详情
WB 1:500 - 1:2000
IF 1:50 - 1:100
Western blot analysis of extracts of various cell lines, using DFFA antibody.
Western blot analysis of extracts from normal (control) and DFFA knockout (KO) HeLa cells, using DFFA antibody.
Immunofluorescence analysis of HeLa cells using DFFA antibody.
背景
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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