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位置: 首页 > 磷酸化抗体 > CDC25A (Phospho-Tyr59) Antibody

CDC25A (Phospho-Tyr59) Antibody#11598

CDC25A (Phospho-Tyr59) Antibody
CDC25A (Phospho-Tyr59) Antibody
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产品总价:
产品详情

产品名称CDC25A (Phospho-Tyr59) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous level of cdc25A only when phosphorylated at tyrosine 59.

免疫原类型Peptide

免疫原描述Peptide sequence around phosphorylation site of tyrosine 59 (S-D-Y(p)-E-Q) derived from Human CDC25A.

基因/蛋白名称CDC25A

修饰Phospho

别名CDC25M3, MPIP1

数据库入口号Swiss-Prot#: P30304
NCBI Gene ID: 993

UniprotP30304

计算分子量70kd

浓度1.0mg/mL

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20˚C

应用详情

Western blotting: 1:500~1:1000

Western blot analysis of extracts from U87 cells, treated with EGF using CDC25A(Phospho-Tyr59) Antibody #11598. Reference: Nat. Commun. 7:12431 doi: 10.1038/ncomms12431 (2016).
Immunohistochemical analysis of a variety of human tumor tissue using CDC25A(Phospho-Tyr59) Antibody #11598. Reference: Nat. Commun. 7:12431 doi: 10.1038/ncomms12431 (2016).
背景

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDC2 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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