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位置: 首页 > 磷酸化抗体 > Beclin1 (Phospho-Ser30) Antibody

Beclin1 (Phospho-Ser30) Antibody#11600

Beclin1 (Phospho-Ser30) Antibody
是否有货: Yes
产品总价:
产品详情

产品名称Beclin1 (Phospho-Ser30) Antibody

来源种属Rabbit

克隆性Polyclonal

应用WB IHC IP

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of Beclin1 only when phosphorylated at serine 30.

免疫原类型Peptide

免疫原描述Peptide sequence around phosphorylation site of serine 30 (D-T-S(p)-F-K) derived from Human Beclin1.

别名ATG6,VPS30,beclin1

数据库入口号Swiss-Prot#: Q14457
NCBI Gene ID: 8678
NCBI mRNA#: NM_003766.4
NCBI Protein#: NP_003757.1

计算分子量52KD

浓度1.0mg/mL

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C

应用详情

 WB dilution: 1:500~1:1000

 IHC dilution: 1:50~1:100

 IP dilution: 1:20~1:50

The indicated His-PGK1 proteins immobilized on Ni-NTA agarose beads was incubated with Flag-ARD1 and Ac-CoA, followed by incubation with purified GST-Beclin1 or GST-Beclin1 S30A, in the presence of [γ- 32P] ATP. Autoradiography was performed.
产品描述

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

背景

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration

背景文献

Xu Qian1,et al. Phosphoglycerate kinase 1 phosphorylates Beclin1 to induce autophagy.Mol Cell. 2017 March 02; 65(5): 917–931.e6. doi:10.1016/j.molcel.2017.01.027. Autho

如果您使用该产品11600发表了文章,请通知我们,让我们可以引用您的文献。

et al,Phosphoglycerate Kinase 1 Phosphorylates Beclin1 to Induce Autophagy.In Mol Cell on 2017 Mar 2 by Xu Qian , Xinjian Li,et al..PMID: 28238651, , (2017),
PMID: 28238651
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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400-9961-933
025-58868422

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