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OLFML2A Antibody#47901

OLFML2A Antibody
OLFML2A Antibody
OLFML2A Antibody
是否有货: Yes
产品总价:
产品详情

产品名称OLFML2A Antibody

来源种属Rabbit

克隆性Polyclonal

纯化The antibody was purified by immunogen affinity chromatography.

应用WB, IHC, IF/ICC

种属反应性Hu,Ms,Rt

特异性Recognizes endogenous levels of OLFML2A protein.

免疫原描述KLH-conjugated synthetic peptide encompassing a sequence within the center region of human OLFML2A.

基因/蛋白名称OLFML2A

别名Olfactomedin-like protein 2A; Photomedin-1

数据库入口号Swiss-Prot#:Q68BL7
NCBI Gene ID:169611

计算分子量73KD

浓度1 mg/ml

配方Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

保存Shipped at 4˚C. Upon delivery aliquot and store at -20˚C for one year. Avoid freeze/thaw cycles.

应用详情

Western blotting:1:500 - 1:1000
Immunohistochemistry:1:50 - 1:100
Immunofluorescence:1:100 - 1:300

Western blot analysis of OLFML2A expression in rat lung (A), mouse lung (B), SGC7901 (C), HEK293T (D), MCF7 (E) whole cell lysates
Immunohistochemical analysis of OLFML2A staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of OLFML2A staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

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Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com