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GAPDH antibody#23001

GAPDH antibody
GAPDH antibody
GAPDH antibody
是否有货: 有货
产品总价:
产品详情

产品名称GAPDH antibody

来源种属Rabbit

克隆性Polyclonal

纯化Purified by antigen-affinity chromatography.

应用WB IHC IF

种属反应性Hu

免疫原类型Recombinant protein

免疫原描述Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 323 of GAPDH

基因/蛋白名称GAPDH

别名G3PD; GAPD; MGC88685

数据库入口号NCBI Gene ID: 2597
NCBI mRNA#: NM_002046
NCBI Protein#: NP_002037

浓度1mg/ml

配方Supplied in 1XPBS, 1%BSA, 20% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

保存Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

应用详情

Predicted MW: 36kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:100-1:200

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
10% SDS PAGE
Primary antibody diluted at 1: 10000
Immunohistochemical analysis of Salivary gland cancer, using GAPDH antibody at 1: 500 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GAPDH antibody (green) at 1: 500 dilution and alpha-tubulin antibody (red) at 1: 2000.
背景

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq]

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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025-58868422

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