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位置: 首页 > 磷酸化抗体 > PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody

PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody#11165

PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody
PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用IHC IF

种属反应性Hu Ms Rt

特异性The Antibody detects endogenous level of PAK1/PAK2/PAK3 only when phosphorylated at threonine 423/402/421.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 423 /402/421 (R-S-T(p)-M-V) derived from Human PAK1/PAK2/PAK3.

基因/蛋白名称PAK1/PAK2/PAK3

修饰Phospho

别名p21-activated kinase 1; PAK-1; p65-PAK; Alpha-PAK;

数据库入口号Swiss-Prot: Q13153/Q13177/O75914
NCBI Protein: NP_001122092.1 NP_002568.2 NP_001121638.1

UniprotQ13153/Q13177/O75914

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 61-67 (PAK2) 68-74 (PAK1/3)kd
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody #11165(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using PAK1/PAK2/PAK3(Phospho-Thr423/Thr402/Thr421) Antibody #11165.
背景

The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.

Parsons M, et al. (2005) Mol Cell Biol ; 25:1680-1695.
Jung JH, et al. (2005) J Biol Chem; 280:40025-40031.
Lampugnani MG, et al. (2002) Mol Biol Cell ; 13: 1175-1189
Wang RA, et al. (2002) EMBO J; 21: 5437 - 5447.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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