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位置: 首页 > 磷酸化抗体 > DNA PKcs(Phospho-Thr2609) Antibody

DNA PKcs(Phospho-Thr2609) Antibody#11167

DNA PKcs(Phospho-Thr2609) Antibody
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产品总价:
产品详情

产品名称DNA PKcs(Phospho-Thr2609) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of DNA PKcs only when phosphorylated at threonine 2609.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 2609 (V-E-T(p)-Q-A) derived from Human DNA-PK.

基因/蛋白名称DNA PKcs

修饰Phospho

别名DNPK1; PRKD; PRKDC; XRCC7; P460

数据库入口号Swiss-Prot: P78527
NCBI Protein: NP_001075109.1

UniprotP78527

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 450kd
Western blotting: 1:500~1:1000
Western blot analysis of extracts from K562 cells untreated(lane 1) or treated with hydroxyurea(lane 2) using DNA PKcs(Phospho-Thr2609) antibody #11167.
背景
The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties

Chan DW, et al. (2002) Genes Dev. Sep 15; 16(18): 2333-2338
Ding Q, et al. (2003) Mol Cell Biol. Aug; 23(16): 5836-5848
Douglas P, et al. (2002) Biochem J. Nov 15; 368(Pt 1): 243-251

如果您使用该产品11167发表了文章,请通知我们,让我们可以引用您的文献。

et al,Pyruvate Kinase M2 Phosphorylates H2AX and Promotes Genomic Instability in Human Tumor Cells.In Oncotarget on 2017 Nov 17 by Li Xia, Kang Qin,et al..PMID: 29312595, , (2017),
PMID: 29312595
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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