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I kappa B-alpha antibody#23045

I kappa B-alpha antibody
I kappa B-alpha antibody
是否有货: 有货
产品总价:
产品详情

产品名称I kappa B-alpha antibody

来源种属Rabbit

克隆性Polyclonal

纯化Purified by antigen-affinity chromatography.

应用WB IF

种属反应性Hu

免疫原类型Recombinant protein

免疫原描述Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 305 of Human NFKBIA

基因/蛋白名称I kappa B-alpha

数据库入口号NCBI Gene ID: 4792
NCBI mRNA#: NM_020529
NCBI Protein#: NP_065390

浓度1mg/ml

配方Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

保存Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

应用详情

Predicted MW: 36kd
Western blotting: 1:500-1:3000
Immunofluorescence: 1:100-1:200

Sample (30 ug of whole cell lysate)
A: Hep G2
10% SDS PAGE
Primary antibody diluted at 1: 1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using IkappaB-alpha antibody at 1: 200 dilution.
背景

NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com