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位置: 首页 > 磷酸化抗体 > a-Synuclein(Phospho-Ser129) Antibody

a-Synuclein(Phospho-Ser129) Antibody#11171

a-Synuclein(Phospho-Ser129) Antibody
a-Synuclein(Phospho-Ser129) Antibody
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产品总价:
产品详情

产品名称a-Synuclein(Phospho-Ser129) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of a-Synuclein only when phosphorylated at serine 129.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 129 (M-P-S(p)-E-E) derived from Human a-Synuclein.

基因/蛋白名称a-Synuclein

修饰Phospho

别名NACP; SYN; SYUA

数据库入口号Swiss-Prot: P37840
NCBI Protein: NP_000336.1

UniprotP37840

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 18kd
Western blotting: 1:500~1:1000
IHC 1:100 - 1:300
IF 1:50-200

Western blot analysis of extracts from mouse brain tissue using a-Synuclein(Phospho-Ser129) Antibody #11171(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Immunohistochemistry analysis of paraffin-embedded human brain, using Synuclein (Phospho-Ser129) Antibody. The picture on the right is blocked with the phospho peptide.
背景

May be involved in the regulation of dopamine release and transport. Soluble protein, normally localized primarily at the presynaptic region of axons, which can form filamentous aggregates that are the major non amyloid component of intracellular inclusions in several neurodegenerative diseases (synucleinopathies). Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.

Takahashi T, et al. J Biol Chem 2003 Oct 24; 278(43): 42225-33
Ahn BH, et al. J Biol Chem 2002 Apr 05; 277(14): 12334-42
Negro A, et al. FASEB J 2002 Feb; 16(2): 210-2
Goldberg, et al. Nat. Cell Biol. 2000; 2, 115-119.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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