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位置: 首页 > 磷酸化抗体 > AMPKα1 (Phospho-Ser496)Antibody

AMPKα1 (Phospho-Ser496)Antibody#11174

AMPKα1 (Phospho-Ser496)Antibody
AMPKα1 (Phospho-Ser496)Antibody
AMPKα1 (Phospho-Ser496)Antibody
AMPKα1 (Phospho-Ser496)Antibody
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产品总价:
产品详情

产品名称AMPKα1 (Phospho-Ser496)Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu

特异性The antibody detects endogenous level of AMPKα1 only when phosphorylated at serine 496.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 496 (S-G- S(P)-V-S) derived from Human AMPKα1.

基因/蛋白名称AMPKa1

修饰Phospho

别名 AAPK1; AMPK alpha-1 chain; AMPK-alpha1; HMG-CoA redustase kinase; PRKAA1

数据库入口号Swiss-Prot: Q13131
NCBI Protein: NP_006242.5

UniprotQ13131

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 63kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKα1 (Phospho-Ser496)Antibody #11174 (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed Hela cells using AMPKα1(Phospho-Ser496)Antibody #11174.
Western blot analysis of extracts from HUVEC cells untreated or treated with UV using AMPKα1 (Phospho-Ser496)Antibody #11174.
Western blot analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPKα1 (Phospho-Ser496) Antibody #11174.
背景

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346.
Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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