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位置: 首页 > 磷酸化抗体 > PLCg2(Phospho-Tyr753) Antibody

PLCg2(Phospho-Tyr753) Antibody#11175

PLCg2(Phospho-Tyr753) Antibody
PLCg2(Phospho-Tyr753) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称PLCg2(Phospho-Tyr753) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of PLCg2 only when phosphorylated at tyrosine 753.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 753 (S-L-Y(p)-D-V) derived from Human PLCg2.

基因/蛋白名称PLCg2

修饰Phospho

别名PLC-IV; PLC-gamma2; Phospholipase C-gamma-2

数据库入口号Swiss-Prot: P16885
NCBI Protein: NP_002652.2

UniprotP16885

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 150kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using PLCg2(Phospho-Tyr753) Antibody #11175.
Immunofluorescence staining of methanol-fixed Hela cells using PLCg2(Phospho-Tyr753) Antibody #11175.
背景
The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.

Kim YJ, et al. (2004) Mol Cell Biol 24: 9986-9999
Humphries LA, et al. (2004) J Biol Chem 279 : 37651-37661
Suzuki-Inoue K, et al. (2004) Biochem J 378 : 1023-1029
Rodriguez R, et al. (2003) Biochem J 374 : 269-280

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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