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UNC13(C. elegans)-like antibody#23107

UNC13(C. elegans)-like antibody
UNC13(C. elegans)-like antibody
UNC13(C. elegans)-like antibody
是否有货: 有货
产品总价:
产品详情

产品名称UNC13(C. elegans)-like antibody

来源种属Rabbit

克隆性Polyclonal

纯化Purified by antigen-affinity chromatography.

应用WB IHC IF

种属反应性Hu

免疫原类型Recombinant protein

免疫原描述Recombinant protein fragment contain a sequence corresponding to a region within amino acids 680 and 927 of human UNC13B

基因/蛋白名称UNC13(C. elegans)-like

数据库入口号NCBI Gene ID: 10497
NCBI mRNA#: NM_006377
NCBI Protein#: NP_006368

浓度1mg/ml

配方Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.

保存Store at -20˚C for long term preservation (recommended). Store at 4˚C for short term use.

应用详情
Predicted MW: 181kd
Western blotting: 1:500-1:3000
Immunohistochemistry: 1:50-1:500
Immunofluorescence: 1:100-1:200
Sample (30 ug of whole cell lysate)
A: 293T
5% SDS PAGE
Primary antibody diluted at 1: 500
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UNC13B antibody at 1: 500 dilution.
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using UNC13B antibody (Green) at 1: 500 dilution and alpha-tubulin antibody (Red) at 1: 2000.
背景
This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq]

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

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Phone :

400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com