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位置: 首页 > 磷酸化抗体 > ASK1(Phospho-Ser966) Antibody

ASK1(Phospho-Ser966) Antibody#11179

ASK1(Phospho-Ser966) Antibody
ASK1(Phospho-Ser966) Antibody
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产品总价:
产品详情

产品名称ASK1(Phospho-Ser966) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu Ms Mk

特异性The antibody detects endogenous level of ASK1 only when phosphorylated at serine 966.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 966 (S-I-S(p)-L-P) derived from Human ASK1.

基因/蛋白名称ASK1

修饰Phospho

别名ASK-1; M3K5; MAP3K5; MAPK/ERK kinase kinase 5; MAPKKK5

数据库入口号Swiss-Prot: Q99683
NCBI Protein: NP_005914.1

UniprotQ99683

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 155kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Western blot analysis of extracts from various cells using ASK1(Phospho-Ser966) Antibody #11179.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1(Phospho-Ser966) Antibody #11179(left) or the same antibody preincubated with blocking peptide(right).
背景

Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.

Zhang W, et al. (2005) J Biol Chem. 280(19): 19036-19044.
Fujii K, et al. (2004) Oncogene. 23(29):5099-5104.
Goldman EH, et al. (2004) J Biol Chem 2004 Mar 12; 279(11): 10442-10449.
Zhang L, et al. (1999) Proc Natl Acad Sci U S A. 96(15): 8511-8515.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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