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位置: 首页 > 磷酸化抗体 > 14-3-3z(Phospho-Ser58) Antibody

14-3-3z(Phospho-Ser58) Antibody#11181

14-3-3z(Phospho-Ser58) Antibody
14-3-3z(Phospho-Ser58) Antibody
14-3-3z(Phospho-Ser58) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称14-3-3z(Phospho-Ser58) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of 14-3-3 z only when phosphorylated at serine 58.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 58 (R-S-S(p)-W-R) derived from Human 14-3-3 zeta.

基因/蛋白名称14-3-3z

修饰Phospho

别名1433Z; 143Z; FAS; Factor activating exoenzyme S; KCIP-1

数据库入口号Swiss-Prot: P63104
NCBI Protein: NP_001129171.1

UniprotP63104

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 28kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200
Western blot analysis of extracts from Hela cells untreated or treated with UV using 14-3-3z(Phospho-Ser58) Antibody #11181.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3z(Phospho-Ser58) Antibody #11181(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using 14-3-3z(Phospho-Ser58) Antibody #11181.
背景
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

Gu YM, et al. (2006) FEBS Lett ; 580(1): 305-310
Powell DW, et al. (2003) Mol Cell Biol; 23(15): 5376-5387
Mackintosh C. (2004) Biochem J; 381(Pt 2): 329-342.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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