Yes
Western blot analysis of Ribosomal Protein L32 expression in
HepG2 (A), NIH3T3 (B), mouse spleen (C), rat ovary (D) whole
cell lysates.
Immunofluorescent analysis of Ribosomal Protein L32 staining
in U2OS cells. Formalin-fixed cells were permeabilized with
0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3%
BSA-PBS for 30 minutes at room temperature. Cells were
probed with the primary antibody in 3% BSA-PBS and
incubated overnight at 4 °C in a hidified chamber. Cells were
washed with PBST and incubated with a Alexa Fluor
594-conjugated secondary antibody (red) in PBS at room
temperature in the dark.
Immunohistochemical analysis of Ribosomal Protein L32
staining in rat brain formalin fixed paraffin embedded tissue
section. The section was pre-treated using heat mediated
antigen retrieval with sodium citrate buffer (pH 6.0). The
section was then incubated with the antibody at room
temperature and detected using an HRP conjugated compact
polymer system. DAB was used as the chromogen. The section
was then counterstained with haematoxylin and mounted with
DPX.
