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位置: 首页 > 磷酸化抗体 > APP(Phospho-Thr668) Antibody

APP(Phospho-Thr668) Antibody#11190

APP(Phospho-Thr668) Antibody
APP(Phospho-Thr668) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称APP(Phospho-Thr668) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of APP only when phosphorylated at threonine 668.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 668 (A-V-T(p)-P-E) derived from Human APP.

基因/蛋白名称APP

修饰Phospho

别名AAA; AD1; PN2; ABPP; APPI

数据库入口号Swiss-Prot: P05067
NCBI Protein: NP_000475.1

UniprotP05067

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 100-140 kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from mouse brain tissue using APP(Phospho-668) Antibody #11190(Lane 2) and the same antibody preincubated with blocking peptide(Lane1).
Western blot analysis of extracts from Hela cells, treated with Noc or calf intestinal phosphatase (CIP), using APP (Phospho-Thr668) Antibody #11190.
背景

APP encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene.

Hung, A.Y. and Selkoe, D.J. (1994) EMBO J. 13, 534-542.
Suzuki, T. et al. (1994) EMBO J. 13, 1114-1122
Ando, K. et al. (1999) J. Neurosci. 19, 4421-4427.
Iijima, K.I. et al. (2000) J. Neurochem. 75, 1085-1091

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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