Yes
Western blot analysis of Frizzled 9 expression in Y79 (A) whole cell lysates.
Immunofluorescent analysis of Frizzled 9 staining in Y79 cells.
Formalin-fixed cells were permeabilized with 0.1% Triton
X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS
for 30 minutes at room temperature. Cells were probed with
the primary antibody in 3% BSA-PBS and incubated overnight
at 4 °C in a hidified chamber. Cells were washed with PBST and
incubated with a DyLight 594-conjugated secondary antibody
(red) in PBS at room temperature in the dark. DAPI was used
to stain the cell nuclei (blue)
