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位置: 首页 > 磷酸化抗体 > HSF1(Phospho-Ser307) Antibody

HSF1(Phospho-Ser307) Antibody#11195

HSF1(Phospho-Ser307) Antibody
HSF1(Phospho-Ser307) Antibody
HSF1(Phospho-Ser307) Antibody
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产品总价:
产品详情

产品名称HSF1(Phospho-Ser307) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IF

种属反应性Hu Ms Rt

特异性The antibody detects endogenous level of HSF1 only when phosphorylated at serine 307.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 307 (P-Q-S(p)-P-R) derived from Human HSF1.

基因/蛋白名称HSF1

修饰Phospho

别名HSTF 1

数据库入口号Swiss-Prot: Q00613
NCBI Protein: NP_005517.1

UniprotQ00613

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 82kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Western blot analysis of extracts from HUVEC cells untreated(lane 1) or treated with TNF-a(lane 2) using HSF1(Phospho-Ser307) Antibody #11195.
Immunofluorescence staining of methanol-fixed Hela cells using HSF1(Phospho-Ser307) Antibody #11195.
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using HSF1 (Phospho-Ser307) Antibody #11195.
背景

DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

Guettouche T, et al. (2005) BMC Biochem ; 6(1): 4
Wang X, et al. (2003) Mol Cell Biol ; 23(17): 6013-6026
Jolly C, et al. (1999) Proc Natl Acad Sci USA; 96(12): 6769-6774.

如果您使用该产品11195发表了文章,请通知我们,让我们可以引用您的文献。

et al,IER family proteIns are regulators of proteIn phosphatase PP2A and modulate the phosphorylation status of CDC25A. In Cell Signal on 2019 Mar by Ueda T, Kohama Y, et al.. PMID: 30599213, , (2019),
PMID: 30599213
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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