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位置: 首页 > 磷酸化抗体 > HSP90B(Phospho-Ser254) Antibody

HSP90B(Phospho-Ser254) Antibody#11196

HSP90B(Phospho-Ser254) Antibody
HSP90B(Phospho-Ser254) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称HSP90B(Phospho-Ser254) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu Ms

特异性The antibody detects endogenous level of HSP90B only when phosphorylated at serine 254.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 254 (V-G-S(p)-D-E) derived from Human HSP90B.

基因/蛋白名称HSP90B

修饰Phospho

别名HS90B; HSP 84; HSP90-beta; HSP90AB1; HSPC2

数据库入口号Swiss-Prot: P08238
NCBI Protein: NP_031381.2

UniprotP08238

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 92kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with hot shock(lane 2) using HSP90B(Phospho-Ser254) Antibody #11196.
Western blot analysis of extracts from Hela cells, treated with calf intestinal phosphatase (CIP), using HSP90B (Phospho-Ser254) Antibody #11196.
背景

Molecular chaperone. Has ATPase activity.

Zhao R, Houry WA.(2007) Adv Exp Med Biol; 594: 27-36.
Olsen JV, et al. (2006) Cell; 127(3): 635-648
Ogiso H, et al. (2004) Biochemistry; 43(49): 15510-15519

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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400-9961-933
025-58868422

Email Address

techcn@signalwayantibody.com