Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-5: 231, PC-3, Hela, A375, HepG2 cell lysates, Primary antibody:COPE Antibody at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds
The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using (COPE Antibody) at dilution 1/50.
背景
The product of this gene is an epsilon subunit of coatomer protein complex. Coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. Coatomer complex consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified.?