产品详情
产品名称EXOSC4 antibody
来源种属Rabbit
克隆性Polyclonal
纯化Antigen affinity purification
应用WB, IHC
种属反应性Hu, Ms
免疫原描述Fusion protein of human EXOSC4
基因/蛋白名称EXOSC4
别名SKI6; p12A; RRP41; Ski6p; RRP41A; Rrp41p; hRrp41p
数据库入口号NCBI Protein#:BC002777
UniprotQ9NPD3
Gene ID54512
计算分子量26 kDa
浓度0.8mg/ml
配方pH7.4 PBS, 0.05% NaN3, 40% Glycerol
保存Store at -20˚C/1 year
应用详情
WB dilution:1:500-1:2000
IHC dilution:1:50-1:200
Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-7: Human fetal liver tissue, RAW264.7, PC-3, 293T, LO2, Hela and Jurkat cell lysates, Primary antibody:EXOSC4 Antibody at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 20 seconds
The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (EXOSC4 Antibody) at dilution 1/50.
背景
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC4 binds to ARE-containing RNAs.