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位置: 首页 > 标记抗体 > Histone H2A.X(Phospho-Ser139) Conjugated Antibody

Histone H2A.X(Phospho-Ser139) Conjugated Antibody#C11268

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产品总价:
产品详情

产品名称Histone H2A.X(Phospho-Ser139) Conjugated Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

种属反应性Hu

特异性The antibody detects endogenous level of Histone H2A.X only when phosphorylated at serine 139.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 139 (Q-A-S(p)-Q-E) derived from Human Histone H2A.X.

标记Biotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750

激发/发射波长AF350: 346nm/442nm AF405: 401nm/421nm AF488: 493nm/519nm AF555: 555nm/565nm AF594: 591nm/614nm AF647: 651nm/667nm AF680: 679nm/702nm AF750: 749nm/775nm

基因/蛋白名称Histone H2A.X

修饰Phospho-Ser139

别名H2A.X; H2AFX; H2a/x; HIST5-2AX;

数据库入口号Swiss-Prot: P16104 NCBI Protein: NP_002096.1

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Suggested Dilution:
AF350 conjugated: most applications: 1: 50 - 1: 250
AF405 conjugated: most applications: 1: 50 - 1: 250
AF488 conjugated: most applications: 1: 50 - 1: 250
AF555 conjugated: most applications: 1: 50 - 1: 250
AF594 conjugated: most applications: 1: 50 - 1: 250
AF647 conjugated: most applications: 1: 50 - 1: 250
AF680 conjugated: most applications: 1: 50 - 1: 250
AF750 conjugated: most applications: 1: 50 - 1: 250
Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1: 50 - 1: 1,000

背景

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Yaneva M, et al. (2005) Nucleic Acids Res. 33(16): 5320-5330.
Tsukuda T, et al.(2006) Nature. Author manuscript; available in PMC 2006 March 6.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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