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位置: 首页 > 磷酸化抗体 > SMC1(Phospho-Ser957) Antibody

SMC1(Phospho-Ser957) Antibody#11198

SMC1(Phospho-Ser957) Antibody
SMC1(Phospho-Ser957) Antibody
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产品总价:
产品详情

产品名称SMC1(Phospho-Ser957) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous level of SMC1 only when phosphorylated at serine 957.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of serine 957 (G-S-S(p)-Q-G) derived from Human SMC1.

基因/蛋白名称SMC1

修饰Phospho

别名SM1A; SMC1A; SMC1L1; SMC1 alpha protein;

数据库入口号Swiss-Prot: Q14683
NCBI Protein: NP_006297.2

UniprotQ14683

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 145kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from 293 cells untreated or treated with UV using SMC1(Phospho-Ser957) Antibody #11198.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using SMC1(Phospho-Ser957) Antibody #11198(left) or the same antibody preincubated with blocking peptide(right).
背景
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

Yang XM. et al. (2004) Mol Cell Biol. 24(12): 5290-5303.
Kim ST. et al. (2002) Genes Dev. 16(5): 560-570.
Revenkova E.et al. (2001) Mol Cell Biol. 21(20): 6984-6998

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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