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位置: 首页 > 磷酸化抗体 > Paxillin(Phospho-Tyr31) Antibody

Paxillin(Phospho-Tyr31) Antibody#11201

Paxillin(Phospho-Tyr31) Antibody
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产品总价:
产品详情

产品名称Paxillin(Phospho-Tyr31) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB

种属反应性Hu

特异性The antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 31.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of tyrosine 31 (T-P-Y(p)-S-Y) derived from Human Paxillin.

基因/蛋白名称Paxillin

修饰Phospho

别名PAXI; PXN;

数据库入口号Swiss-Prot: P49023
NCBI Protein: NP_001074324.1

UniprotP49023

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情

Predicted MW: 68kd
Western blotting: 1:500~1:1000

Western blot analysis of extracts from HT29 cells untreated or treated with PMA using Paxillin(Phospho-Tyr31) Antibody #11201.
背景

An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions

Davidson D, et al. (2001) EMBO J 20(13): 3414-3426.
Fleming I, et al. (1999) Proc Natl Acad Sci U S A 96(3): 1123-1128.
Goldberg MB. (2001) Microbiol Mol Biol Rev 65(4): 595-626.
Kook S, et al. (2000) Mol Biol Cell 11(3): 929-939.

如果您使用该产品11201发表了文章,请通知我们,让我们可以引用您的文献。

et al,Discovery of a novel inhibitor of the protein tyrosine phosphatase Shp2.In Sci Rep on 2015 Dec 2 by Chuan Chen , Mengmeng Cao et al..PMID: 26626996 , , (2015),
PMID: 26626996
et al,Lipid raft regulates the initial spreading of melanoma A375 cells by modulating 尾1 integrin clustering. In Int J Biochem Cell Biol on 2013 Aug by Ruifei Wang, Jiajia Bi,et al..PMID: 23665237, , (2013),
PMID: 23665237
Zhi Huang, Da-Peng Yan, Bao-Xue Ge et al,JNK regulates cell migration through promotion of tyrosine phosphorylation of paxillin., Cellular Signalling , 20(11): 2002-2012 (2008),
PMID: 18713649
et al,JNK regulates cell migration through promotion of tyrosine phosphorylation of paxillin.In Cell Signal on 2008 Nov by Zhi Huang , Da-Peng Yan, et al..PMID:18713649, , (2008),
PMID: 18713649
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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