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位置: 首页 > ELISA试剂盒 > Human TSLP ELISA kit

Human TSLP ELISA kit#EK0479

Human TSLP ELISA kit
是否有货: 1-2周
产品总价:
产品详情

产品名称Human TSLP ELISA kit

简述ELISA Kit

应用ELISA

种属反应性Hu

特异性Natural and recombinant Human TSLP Ligand

交叉反应性No significant interference observed with available related molecules.

基因/蛋白名称Human TSLP

应用详情
Detect Range: 3.9-250pg/ml
Sensitivity: 2pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric
Representative standard curve for TSLP ELISA. TSLP was diluted in serial two-fold steps in Sample Diluent.
产品描述
  • Aluminium pouches with a Microwell Plate coated with antibody to human TSLP (8X12)
  • 2 vials human TSLP Standard lyophilized, 500 pg/ml upon reconstitution
  • 2 vials concentrated Biotin-Conjugate anti-human TSLP antibody
  • 2 vials Streptavidin-HRP solution
  • 1 bottle Standard /sample Diluent
  • 1 bottle Biotin-Conjugate antibody Diluent
  • 1 bottle Streptavidin-HRP Diluent
  • 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
  • 1 vial Substrate Solution
  • 1 vial Stop Solution
  • 4 pieces Adhesive Films
  • package insert
背景

Thymic Stromal Lymphopoietin (TSLP) is a 23 kDa member of the IL-7 family of a-helical cytokines (1-3). It is a monomeric glycoprotein that is synthesized as a 159 amino acid (aa) precursor (4, 5). TSLP appears to have species-specific functions. In mouse, TSLP was initially reported to act on NK cells, mast cells, and B cells, but this does not appear to occur in humans. In humans, TSLP is produced by a number of divergent cell types, all of which appear to target T cells, monocytes, and/or dendritic cells (2). On TCR-activated T cells, TSLP directly induces T cell proliferation (6). The significance of this direct action is unclear. On monocytes, TSLP is reported to induce the release of multiple chemokines that target CCR4, a receptor associated with the Th2 subset (5). TSLP is best known for its direct action on subsets of dendritic cells. In thymic medulla, Hassell

背景文献

Liu, Y-J. et al. (2007) Annu. Rev. Immunol. 25:193.

Liu, Y-J. (2006) J. Exp. Med. 203:269.

Ziegler, S.F. and Y-J. Liu. (2006) Nat. Immunol. 7:709.

Quentmeier, H. et al. (2001) Leukemia 15:1286.

Reche, P.A. et al. (2001) J. Immunol. 167:336.

Rochman, I. et al. (2007) J. Immunol. 178:6720.

Watanabe, N. et al. (2005) Nature 436:1181.

Ebner, S. et al. (2007) J. Clin. Allergy Immunol. 119:982.

Soumelis, V. et al. (2002) Nat. Immunol. 3:673.

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5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

联系我们

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025-58868422

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