• Aluminium pouches with a Microwell Plate coated with antibody to Swine IL-4 (8X12)
• 2 vials Swine IL-4 Standard lyophilized, 5000 pg/ml upon reconstitution
• 2 vials concentrated Biotin-Conjugate anti-Swine IL-4 antibody
• 2 vials Streptavidin-HRP solution,
• 1 bottle Standard /sample Diluent
• 1 bottle Biotin-Conjugate antibody Diluent
• 1 bottle Streptavidin-HRP Diluent
• 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
• 1 vial Substrate Solution
• 1 vial Stop Solution
• 4 pieces Adhesive Films
• package insert

Interleukin4 (IL-4), also known as B cellstimulatory factor1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-3).It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four αhelix structure (4). Porcine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature porcine IL-4 shares 78%, 59%, 41%, and 41% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human IL-4 is active on porcine vascular endothelial cells (5). IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 Rα and the common γ chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4Rα and IL13Rα1. The type II receptor also transduces IL-13 mediated signals. IL4 is primarily expressed by Th2biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by na?ve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8, 11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.

Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.

Bailey, M. et al. (1993) Biochim. Biophys. Acta 1171:328.

Redfield, C. et al. (1991) Biochemistry 30:11029.

Stocker, C.J. et al. (2000) J. Immunol. 164:3309.

Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.

Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.

Paludan, S.R. (1998) Scand. J. Immunol. 48:459.

Corthay, A. (2006) Scand. J. Immunol. 64:93.

Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.

Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.

Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.