产品详情
产品名称Chk2 Antibody
来源种属Rabbit
克隆性Polyclonal
纯化Affinity chromatography purified via peptide column
应用ELISA WB ICC
种属反应性Hu Ms Rt
免疫原类型Peptide
免疫原描述Raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2.
基因/蛋白名称Chk2
数据库入口号NP_009125
浓度1mg/ml
配方Supplied in PBS containing 0.02% sodium azide.
保存Can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
应用详情
Predicted MW: 60 kd
Western blot analysis of Chk2 expression in K562 (A), Jurkat (B), and HL-60 (C) whole cell lysates with Chk2 antibody at 1 ug /ml.
Immunocytochemistry of Chk2 in Jurkat cells with Chk2 antibody at 1 ug/mL.
Immunofluorescence of Chk2 in Jurkat cells with Chk2 antibody at 5 μg/mL
背景
The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2 , p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer.
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