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位置: 首页 > 磷酸化抗体 > p27Kip1(Phospho-Thr187) Antibody

p27Kip1(Phospho-Thr187) Antibody#11208

p27Kip1(Phospho-Thr187) Antibody
p27Kip1(Phospho-Thr187) Antibody
是否有货: 有货
产品总价:
产品详情

产品名称p27Kip1(Phospho-Thr187) Antibody

来源种属Rabbit

克隆性Polyclonal

纯化Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

应用WB IHC

种属反应性Hu

特异性The antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187.

免疫原类型Peptide-KLH

免疫原描述Peptide sequence around phosphorylation site of threonine 187 (E-Q-T(p)-P-K) derived from Human p27Kip1.

基因/蛋白名称p27Kip1

修饰Phospho

别名CDKN1B; CDN1B; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; KIP1

数据库入口号Swiss-Prot: P46527
NCBI Protein: NP_004055.1

浓度1.0mg/ml

配方Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

保存Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

应用详情
Predicted MW: 27kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Western blot analysis of extracts from Hela cells untreated or treated with EGF, IFN-a using p27Kip1(Phospho-Thr187) Antibody #11208.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p27Kip1(Phospho-Thr187) Antibody #11208(left) or the same antibody preincubated with blocking peptide(right).
背景
Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.

Eguchi H, et al. (2003) Cancer Res; 63(15): 4739-46
Le XF, et al. (2003) J Biol Chem;
Connor MK, et al. (2003)

如果您使用该产品11208发表了文章,请通知我们,让我们可以引用您的文献。

et al,Insights in dynamic kinome reprogramming as a consequence of MEK inhibition in MLL-rearranged AML.In Leukemia on 2014 Mar by K R Kampen, A Ter Elst et al..PMID:24240200, , (2014),
PMID: 24240200
5

注释

应用

  • WB免疫印迹
  • IHC免疫组化
  • IF免疫荧光
  • ICC免疫细胞化学
  • FC流式细胞
  • IP免疫沉淀
  • E酶联免疫吸附法
  • DB免疫斑点法
  • ChIP染色质免疫沉淀
  • GICA胶体金免疫层析法
  • NC阴性对照

种属反应性

  • Hu
  • Ms小鼠
  • Rt大鼠
  • Dm果蝇
  • C线虫
  • Mk
  • Rb
  • B
  • D
  • P
  • Hm仓鼠
  • ChHm中国仓鼠
  • Chk

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